For the purpose of studying molecular mechanism of some narcotic substances´ action we have carried out experiments on bilayer phospholipid membranes with anion-selective channels formed by amphotericin B. It has been found out that 5.10-4 М of cocaine doubled the conductivity of such membranes without affecting that of the unmodified phospholipid bilayers´. It has been demonstrated that anaesthetics are ranged - cocaine, lidocaine, novocaine, - according to their pharmacological action series. It has been supposed that the molecular mechanism of the detected effect is connected with the action of anaesthetics on the lipid bilayer surface charge. The following anaesthetic gases were tried by us on biological and artificial membranes: Halothane, Methoxyfluranum, Chloroform and Butanol. It has been established that some compounds, local and general anaesthetics among them, cause the orderliness factor contraction or, in other words, dissolve the membranes. It was shown that the membrane should be in a certain optimal state to function well. After the inhalant addition the membrane resistance began to fall and after 15-20 min achieved a new level. At that the conductivity increased by 1, 6-3 orders more. It was suggested that perhaps the membrane dissolution accelerated their interaction. This assumption was verified while determining the time of two phospholipids membranes´ fusion, when the anaesthetic was added into the solution 10mM KS1, wherein the work was carried out. The data obtained show that in the presence of the investigated inhalants the membranes´ fusion accelerated several times as much. The membrane resistance reduction, which occurs in the presence of inhalants, doesn´t influence their fusion by itself. It was shown on the model membranes that cocaine doubles the penetrance of phospholipid membranes with anion-selective channels, and inhalation anaesthetics of narcotic action accelerate their fusion several times, when dissolving the membranes.
On the m. Cutaneus pectoris Rana temporaria nerve-muscle preparation the influence of some local anaesthetics (LA) (norcaine, novocaine, viadril, trimecaine, lidocaine and its analogues QX-314 и QX-572) was studied. All of them possess a postsynaptic action, the miniature endplate potentials´ amplitude contraction (MEP) testifying to this fact. Proceeding from the data obtained one can conclude that all the investigated LA promote the emission of Са2+ ions into the nerve terminal protoplasm. The LA blocking effect, which manifests itself as the MEP amplitude contraction, happens due to the interaction of LA cationic type (i.e. quaternary amine) with the anionic receptors of the electro-excited membranes´ sodium channel orifice.
The work was submitted to international scientific conference «Innovation technologies», USA, (New York) December, 19-27, 2007, came to the editorial office 26.10.07.