Angiogenesis is a process of the new vessel generation from the existing vascular bed. It is typical for a tumor progression. The increase the invasiveness and metastatic activity of the neoplasm is a result of the angiogenesis.
The parameters of the vascular bed were studied based on the operational bioptic material of the primary tumor of 83 ovarian cancer (OC) patients.
It was found that in primary OC tumors vessels are distributed very irregularly. They have poorly developed junctional stroma and thin walls. Very rarely the vessels are covered directly by neoplasm. The considerable increase in number of the bundle vessels, hyperinflated and hyperemic vessels can be found in the marginal layer of the tumor.
The main amount of vessels of the tumor microcirculature is capillary vessels with the diameter more than 10 µm (Table 1).
The endothelial cover of the tumor stroma in small sinuses are represented by one or two endothelial cells the nuclei of which emerge in a vessel lumen and cytoplasmic outgrowths embrace the vessel on its perimeter forming a solid tube. At the same time the basal membrane is often not recognized or distinguished as a discrete plate. The increase of a caliber of the new-formed vessels is not accompanied by their structural alteration: even large diameter vascular walls are close to the structure of the capillary vessel walls.
Table 1. Parameters of the tumor vascular bed
|
Vascular parameter |
М |
m |
V.c. |
Max |
Min |
|
Volume, % |
4,33 |
0,25 |
0,46 |
12,6 |
1,2 |
|
Number in 1 mm2 of the tumor |
23,7 |
1,1 |
0,38 |
62,2 |
12,1 |
|
Length in 1 mm3 of the tumor, mm |
423,3 |
37,4 |
0,72 |
1354,0 |
25,4 |
|
Wall square in 1 mm3 of the tumor, mm2 |
14,5 |
1,22 |
0,7 |
77,0 |
2,2 |
|
Diameter 5-10 µm, % |
16,2 |
2,17 |
0,29 |
100,0 |
0 |
|
Diameter 11-20 µm, % |
43,4 |
1,25 |
0,47 |
58,8 |
0 |
|
Diameter 21-30 µm, % |
27,3 |
0,95 |
1,16 |
40,8, |
0 |
|
Diameter 31-50 µm, % |
6,8 |
0,3 |
1,57 |
12,8 |
0 |
|
Diameter 51-100 µm, % |
4,1 |
0,18 |
2,77 |
8,5 |
0 |
|
Diameter 101-200 µm, % |
2,2 |
0,08 |
2,95 |
3,9 |
0 |
Therefore, the investigation of the vascular density on 1 mm2 of the tumor, the ratio of their diameters, volume of vessels, the length and square of vascular walls in 1 mm3 of tumor tissue showed in average the high level of blood supply in the primary tumor knot in the ovarian cancer, though in particular cases the parameter variations were significant.
The article is admitted to the III International Scientific Conference «Present-day problems of experimental and clinical medicine», Thailand (Bangkok, Pattaya), December 19-27, 2007, came to the editorial office on 31.10.07