The serum samples of 205 women served as the research material. Among them there were 45 samples with the gastric cancer (GC) histological diagnosis, 70 - with intestinal cancer (IC) and 90 healthy samples. Every woman gave a written agreement to take part in the research. The content of antibodies (AB) to benzo[a]pyrene (BP) was determined by means of the modified by us method ELISA. The statistic treatment of the results was carried out with the help of standard methods.
In the majority of the researched serum samples we managed to find out the AB to BP of all three classes (IgA, IgM, IgG). Authentic differences were detected on the BP AB levels of the classes A and G between the healthy and GC patient women, and also between the healthy women and IC patient ones. The fact, that in the healthy women the specific gravity of serum samples, in which no BP AB of the A class were detected, turned out to be the highest one (8,3%), comes under notice. On the content of the BP AB of the M class the compared groups didn´t differ.
There were no differences on the BP AB levels of all three classes detected between the patients in different stages of the tumor process, and also between the GC and IC patients.
Thus, the BP AB are formed both in the healthy and GC and IC patients. At that, the levels of BP AB of the A and G classes in the considered localizations cancer patients are higher than in healthy women.
The fact, that BP AB are detected not only in malignant tumor patients, but also in the majority of healthy women, is of great interest as well.
In our opinion, to establish a cancerogenes AB critical level, the exceedence of which could be estimated as the sign of cancerogene-protein adducts quantity increase, i.e. as the factor of an individual carcinogenic risk, is extremely important to all practical purposes. For this particular purpose we have analyzed the variational series of the A, M and G classes BP AB content in the healthy women group and on the basis of the t criterion defined the affinity of utmost variants to the general aggregate of the factors. We relatively accepted the BP AB quantity maximum value, higher of which the aggregate variants "fall out" of the variational series on the t criterion, for the upper limit of normal. 4,6 mcg/ml for the A class BP AB, 21,8 mcg/ml - for the G class ones, 38,7 mcg/ml - for the M class - turned out to be such limits.
It has been found that the number of women, in which the A and G class BP AB content exceeds the relative limit of norm, among the GC and IC patient ones is authentically higher (on the c2 criterion) than that among the healthy women. There were no differences on the M class BP AB content detected.
Conclusions
- In blood serum of healthy women there are antibodies to BP.
- In GC and IC patients the content of AB to BP is higher, than in healthy women.
- The appearance of AB to BP at GC and IC has specific isoallotypic features: at GC and IC the A and G classes´ antibodies content increases preferentially.
- An increased content of AB to BP can be a sign of a high oncorisk, but the lack of AB or their low content in the serum is not the sign of a low oncorisk.
- The content of AB to BP in GC and IC patients doesn´t depend on the stage of the tumor disease.
The article is admitted to the International Scientific Conference «Advances in current natural sciences», Moscow, May 13-15, 2007, came to the editorial office on 15.11.07