Scientific journal
European Journal of Natural History
ISSN 2073-4972
ИФ РИНЦ = 0,301

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ENZYMIC PROFILE IN PATIENTS WITH NEURODYSTROPHIC FORM OF LUMBAR ISCHIALGIA AND OSTEOCHONDROSIS OF LUMBAR SPINE

Peresypkin V. and Peresypkin M.
It is known that every fifth inhabitant of our planet aged after 30 suffers from these or those presentations of spinal osteochondrosis, and at the age of 60 and older spinal osteochondrosis appears in almost 100% of cases (Antonov I.P., 1985). Osteochondrosis occupies first place among reasons of temporary disablement (Yumashev G.S. and etc., 1984). Moreover, immune-biochemical blood status in comparison to other internal diseases is not enough studied and pathogenetic mechanisms of osteochondrosis and the pain syndromes are in many ways unknown.

Aim of the work: to study activity of some enzymes of purine metabolism and antioxidant hemic system in patients with neurodystrophic form of lumbar ischialgia and osteochondrosis of lumbar spine.

Material and methods. There were 47 patients with neurodystrophic form of lumbar ischialgia and osteochondrosis of lumbar spine. The diognosis was verified on the basis of anamnesis, complaints, data of clinical examination and roentgenologic, functional and reovasographic testing results. According to Russian classification of osteochondrosis of lumbar spine (Veselovsky V.P., 1977) all patients were detected neurodystrophic form of lumbar ischialgia (special gathering of patients). Men dominated among the patients (72,3%). Average age -43,7+ 1,1 years, desease duration - 5,28 +_ 0,2 years. Progredient type was detected with 16 patients, stable - with 25 and regredient - with 6 patients.

Enzym activity: xanthine oxidase (XO), xanthine dehydrogenase (XDH), guanase (G), purinenucleosidephosphorylase (PNP), adenosine desaminase (ADA), adenosine monophosphate- deaminases (AMPDA), superoxide dismutase (SOD), glutathione peroxidase (GP), glutathione reductase (GR), content of malondialdehyde (MDA), uric acid (UA) were detected in blood serum and hemolysate with the help of standard methods (Caraway W., 1966; Martinek R., 1963; Robertson B. et al., 1973; Lankin V.I. and others 1983; Chevari S. and others 1985).

Research results. In blood serum of healthy people activity of ADA was 8,02±0,16 IU, AMPDA -1,98±0,12 IU, G -1,27±0,13 IU, PNP - 0,76±0,07 micromole/l/min, XO - 3,75±0,06 micromole/l/min, XDH - 5,81±0,1 micromole/l/min, SOD in erythrocytes 36,9±1,5 units, SOD in plasma- 5,15±0,09 units, GP in erythrocytes 141,1±5,98 units, GP in plasma -0,97±0,04 units, GR in erythrocytes- 141,1±5,98 units, GR in plasma -1,69±0,06 units, content of MDA 3,46±1,08 nmole/ml, UA - 0,29±0,01 mmole/l.

In patients with neurodystrophic form of lumbar ischialgia it was detected in blood serum: increased activity G (р<0,05), PNP (р<0,001), XO (р<0,001), GP in plasma (р<0,001), decreased activity ADA (р<0,001), AMPDA (р<0,001), SOD in erythrocytes (р<0,05), SOD in plasma (р<0,05), GR in plasma (р<0,01), increased content of MDA (р<0,05) and UA (р<0,05). Progredient type of course was characterized by the increase of activity in blood serum of G (р<0,05), PNP (р<0,001), XO (р<0,001), content of MDA (р<0,05), UA (р<0,05), in blood serum decrease of activity ADA (р<0,001), AMPDA (р<0,001), XDH (р<0,05), SOD in erythrocytes (р<0,01) and GR in plasma (р<0,01). In stable course activity ADA (р<0,001), AMPDA (р<0,05), GR in plasma (р<0,5)is lower in comparison to healthy ones and activity PNP (р<0,001), XO (р<0,001), GP in plasma (р<0,001) is higher. In regredient course only activity XDH in blood serum was higher (р<0,05). In patients with progredient course activity in blood serum ADA (р<0,001), AMPDA (р<0,001), SOD in erythrocytes (р<0,001) and GR in plasma (р<0,05) was lower in comparison to stable course, but activity PNP (р<0,001), XO (р<0,001), MDA level (р<0,001), UA (р<0,01) was higher. In comparison to regredient course activity in blood serum G (р<0,05), PNP (р<0,001), XO (р<0,01), MDA level (р<0,001), UA (р<0,001)was higher, activity ADA (р<0,001), AMPDA (р<0,001), SOD in erythrocytes (р<0,001), SOD in plasma (р<0,05) and GR in plasma (р<0,05) was lower. In patients with stable course activity in blood serum G (р<0,05), PNP (р<0,01), was higher in comparison to regredient, but activity ADA (р<0,01), AMPDA (р<0,001), XDH was lower.

Conclusion. The undertaken research of patients with neurodystrophic form of lumbar ischialgia detected the decreased activity of enzymes of antioxidant blood system, strengthening of the lipid peroxidation process, catabolism of purine bases and activity increase of proinflammatory enzyme - XO, conducing to hyperproduction of superoxide radical that may be one of the pathogenetic mechanisms of osteochondrosis of lumbar spine. The studied enzyme blood data conduce to specification of the character of disease course and ordering suitable therapy.

The work is presented for an International Science Conference "Contemporary issues of experimental and clinic medicine". Thailand (Bangkok-Pattaya), December  20-30, 2009. Received by the editorship on 11.11.2009.