Scientific journal
European Journal of Natural History
ISSN 2073-4972
ИФ РИНЦ = 0,301

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ERYTHROCYTES’ MORPHOMETRIC ANALYSIS AT THE ELDERLY SUFFERING FROM PANCREATIC DIABETES

Lipunova E.A., Skorkina M.Y., Tukin V.N.
At diabetes (D) it is the erythrocytic component that suffers the reaction of activity increase of free radical oxidation first and is the first to exhaust its compensatory possibilities (E.V. Roytman and co-authors, 2001). However, red blood cells´ changes in metabolic disturbance complex associated with D are studied not sufficiently, that is becoming the reason of not always adequate metabolic care (Bondar T.P. and co-authors., 2002). The aim of the research was to study the morphometric characteristics of erythrocytes of the elderly (men and women) suffering from D.

28 persons (15 men of 50,5±3,6 years old and 13 women 56,9±2,5 years old) having suffered from insulin-dependent D of 2 type for 8,6±1,9 and 12,2±2,9 years accordingly, were examined; their hematologic indexes being studied and the erythromorphometry being carried out.

The hematologic indexes of clinically healthy men and women didn´t exceed the bounds of the conditional physiological norm. The comparative analysis revealed a higher value of erythrocytes in a blood volume unit by 14, 7%, hemoglobin - by 14, 8%, hematocrit index - by 14, 6% (p<0, 05) at the men. The mean cell value and hemoglobin concentration didn´t have authentic differences, but the tendency to higher indexes at the men was marked. Comparing erythromorphometric characteristics of healthy patients we noted that the average diameter (by 6, 3%) and average area of a cell surface (by 5, 8%) were more and the thickness index (by 11, 1%) less at the men; the mean cell volume of red blood cells didn´t have authentic differences.

The diabetic patients´ red blood indexes were in the bounds of the norm as well, but compared to the control group a more lower value of total and mean cell hemoglobin by 11, 9 and 7, 6, and 17, 8 and 8, 0 % at the men and the women accordingly. The fundamental characteristic of an erythrocyte is volume: at the diabetic patients it was lower - 94,2±0,4 and 84,3±2,34 мкм3, and 94,3±0,9 and 88,9±2,3 мкм3 (p<0,01) at the healthy and sick men and women accordingly. In the diabetic patients´ groups the diameter-thickness ratio was authentically higher and the specific surface area was lower, the differences being graded more vividly at the women than at the men.

Conclusions:

  1. blood of clinically healthy men contains more erythrocytes and hemoglobin in a blood unit volume than that of women, and it has a more higher hematocrit index;
  2. sex specificity of the main hematologic indexes retains at the diabetic patients;
  3. specific and average surface areas and the average volume of an erythrocyte is lower , but diameter-thickness ratio is higher at the patients;
  4. geometric profile changes of red blood cells decreases their contribution in normal functioning of homeostatic systems of a body at diabetes;
  5. hematologic profile and morphometric characteristics´ changes of erythrocytes at diabetic patients are valued as adaptive processes aimed at blood flow properties perfecting and reducing tissue hypoxia in erythron system.

The article is admitted to the V International Scientific Conference "Modern High Technologies"; Egypt, Hurgada, February, 21-28, 2007; came to the editorial office on 11.01.07