For this purpose the following cultures were used: Proteus mirabilis, Pseudomonas aeruginosa, Klebsiella spp., Citrobacter diversus, Staphylococcus aureus. Cultures were grown on meat peptone agar. The filter disks soaked with 1%, 5% sodium humat solution were placed in a Petri dish with culture and then into a thermostat at 37◦ C. The findings were counted after 24 hours. The experiments showed that the agent had a bacteriological effect.
Rats were used for in vitro experiments. Incised flat wounds were made on their backs to make an experimental model of bacterial infection. One ml of 18-hour cultures grown on Hottinger broth (pH 7,2 - 7,4) with an optic density of 100 mln - 1 mld of colony forming units was placed on the wound. After a 4-day perid, suppurative wounds of the experimental animals were treated with the ointment containing sodium humat at a concentration of 1%, 5% as well as a polyethyleneglycol ointment basis and synthomycin emulsion. After 3-7 days following the wound treatment with sterile tampons, the purulent material was taken and seeded in universal and differential-diagnostic media. Clean cultures were isolated and identified according to their cultural, morphological and biochemical properties.
It has been established that 1%, 5% ointment with sodium humat similar to the synthomycin emulsion has antimicrobial activity as related to Proteus mirabilis, Pseudomonas aeruginosa, Klebsiella spp., Citrobacter diversus, Staphylococcus aureus. The wound surfaces became pus free during a shorter period of time as compared to control animals. The wound healing was faster in experimental groups in comparison with control rats.
The article is admitted to the IV International Scientific Conference "Modern Problems of Experimental and Clinical Medicine", Thailand, 2007, January 17-28; came to the editorial office on 14.11.06