Scientific journal
European Journal of Natural History
ISSN 2073-4972

DYNAMICS OF CONTENTS OF GROWTH FACTORS, CYTOKINES OF PRO-INFLAMMATORY EFFECT IN SYNOVIAL FLUID AMONG PATIENTS WITH OSTEOARTHRITIS AGAINST THERAPY

Knyazeva L.A. 1 Brezhneva I.N. 1 Goryainov I.I. 2 Lukashov A.A. 1 Maltseva G.I. 1
1 Kursk State Medical University
2

The objective of this work is to study an impact of zinaxin upon contents of growth factors: vascular endothelial growth factor A (VEGF-A), fibroblast growth factor (FGF), and pro-inflammatory cytokines (interleukin-1b, IL-1b, IL-6, factor of tumour necrosis α – FTN-α) in synovial liquid of patients with osteoarthritis (OA) against treatment with zinaxin.

Methods and materials. We have studied 65 patients with OA. Among those – 18 men (27,7 %) and 48 women (72,3 %). An average age oscillated from 46 to 68 years. Diagnosis OA was established according to diagnostic criterions EULAR (2010). A control group was formed of 20 healthy donors. Synovial liquid for examination was received via puncture of ancles. LevelofIL-1b, IL-6, FTN-α wasde fined byaimmune-fermenine method using test-systems «Protein contour» (Sankt-Petersburg), VEGF-А – by Bender Med systems Gmbh (Austria) andFGF – by Biosource Gmbh (Belgium). Study of contents of pro-inflammatory cytokines and growth cytokines in synovial liquid among patients with OA was carried out before therapy and in 6 months after treating them with zinaxin. Statistical analysis of the received data was carried out with programme complex Statistica 8,0 for Windows.

Results and discussions. The results of defining initial level of pro-inflammatory cytokines in synovial liquid among patients with OA have shown a reliable increase in concentration of IL-1b 1,8 ± 0,2 times (p < 0,05), IL-6 2,2 ± 0,3 times (p < 0,05), FTN-α 2,3 ± 0,4 times (p < 0,05), compared to the control, level of IL-1b in which equaled – 15,4 ± 6,1 pg/ml; IL-6 – 6,2 ± 1,8 pg/ml; FTN-α – 32,6 ± 4,4 pg/ml. Defining level of VEGF-А in synovial liquid under OA showed an increase in its concentration by 7,7 ± 0,7 (p < 0,01), compared to the control (15,6 ± 3,6 pmole/ml). Within our research we have also studied contents of FGF in synovial liquid of patients with OA that showed an increase in level of FGF 1,4 ± 0,3 times, compared to the control (5,1 ± 0,6 pg/ml, p < 0,05).

Studying dynamics of laboratory indications in 6 months after the taken therapy with zinaxin showed a decrease in average level of:IL-1b – by 43,8 ± 1,9 % (p < 0,05), IL-6 – by 45,9 ± 1,4 % (p < 0,05), FTN-α – by 35,9 ± 1,8 % (p < 0,05), VEGF-Аby 28,4 ± 1,4 % (p < 0,05), FGF by 26,4 ± 1,2 % (p < 0,05), compared to the initial data.

Resume. An increase in level of pro-inflammatory cytokines (IL-1b, IL-6, FTN -α) and growth factors (VEGF-А, FGF) in synovial liquid is registered among patients with OA. Zinaxin has a resolving effect over contents of IL-1b, IL-6, FTN-α, VEGF-А, FGF in synovial liquid among patients with osteoarthritis.

The work was submitted to International Scientific Conference «Fundamental and applied research in medicine», France (Paris), 14-21, October, 2012, came to the editorial office on 18.10.2012.