Scientific journal
European Journal of Natural History
ISSN 2073-4972

THE CONTENT OF THIOLIC GROUPS IN MITOCHONDRIA OF DIFFENENT PIG ORGANS

Dementyeva T.A.

The concentration of sulfhydryl groups in blood and tissues reflects the intensity of metabolism. The involvement of thiolic groups in the processes of cell division and growth is identified. The SH-groups manifest their very good ability to enter into reactions. Thiols have hydrophilic properties. The thiols become localized in an aqueous phase of a cell where they protect a great many biologically important molecules from oxidative damages.

An experiment was carried out on the SSF experimental training farm «Tulinskoye» of Novosibirsk State Agrarian University. Kemerovo pigs were the objects to examine. The animals were selected for the principle of analogues. Biochemical indexes of the blood of 6-month pigs were examined. The content of SH-groups was examined in mitochondria, liver and heart supernatant in the pigs. Tissue samples were taken from 6 animals of each group during control slaughtering. Mitochondria were isolated out of 10 % homogenate in the 0,25 M solution of sucrose with the method of differential centrifuging. The mitochondrial fraction purity was tested by contrasting phases. To analyze, there was taken the number of mitochondria corresponding to 0,1-0,2 mg of mitochondrial protein. The protein was determined with bull serum albumin as a standard.

Statistical processing of the data was done with the package of applied software Statistica 6 and Excel.

The examinations showed that the concentration of general SH-proups in mitochondria of pig liver was twice as much as pig heart of both Large White and Kemerovo breeds. Regarding the supernatant, the differences were somewhat less. The experiment identified the increased concentration of the general SH-groups in liver mitochondria in Kemerovo gilts (0,01) versus Large Whites. The same age Kemerovo pigs exceeded Large Whites by 10,98 % (0,05) for the level of thiols titer in heart mitochondrial fraction. The experimental data allow to suggest high anti-oxidative activity of thiol groups in mitochondria of different organs in Kemerovo breed.

 


The work was submitted to international scientific conference «Fundamental research», Israel (Tel Aviv), 16-23 October 2011, came to the editorial office on 19.10.2011.