History of the biocrystallization phenomenon scrutiny includes over 30 years [1, 3, 4], but the first mention about it had been written in 1804 . There are many papers, which demonstrated diagnostic function of crystalloscopic and tezigraphic facia . In that time, the unitary publications devote to ability of crystallographic methods for indication of treatment effectiveness [1, 3]. This thesis was aim of our investigations.
Materials and methods. We study the dynamics of the transformation of teziocrystalloscopic picture of some human biofluids (saliva, urine, blood serum, teardrops etc.) in the management process. Free crystallization of biomaterial (classic crystalloscopy) was examined by using the special identification table, which consist of 5 classes of crystal and amorphous structures and half-quantitive additional criteria, such as facia destruction degree [FDD], regularity [R], cellularity [C] and marginal belt [MB]. Tezigraphic facia was evaluated by complex of basic (initiation coefficient [IC]; belt coefficient [BC]) and additional parameters [1, 3]. We used two variants of tezigraphic test. There are comparative and differential teziography, which discrepant by number of the basic substances. Data were processed with statistic programs (SPSS 11.0; Primer of biostatistics 4.03).
Results. On the base of our data it was demonstrated, that the dynamics of the biofluids¢ teziocrystalloscopic picture correlates with patient common condition and his clinic-functional status. This thesis was verificated on patients, which have gastroenterological, neurological, traumatologinal, cardiological and nephrological diseases. We tested the dynamics of free and initiated biosubstrates crystallogenesis on medicamental, chirurgical, balneological and physiotherapeutic management. On our opinion, the most informative and comfortable for practical using biosubstrates are saliva and urine, but informativity of the monitoring highly increase, if two or more biofluids are analysed simultaneously. It was shown, that character of the crystalloscopic specimen´s changes (on the general tendency to organization or destruction of the facia) illustrates the treatment effectiveness. This conclusion with respect general tendency is formed by the analysis of crystallization rate (crystal concentration in microscope visual field), facia destruction degree, homogeneity of elements allotment, cellularity, marginal zone width etc. It is determined, that positive treatment results associate with decreasing of facia destruction degree, cellularity; increasing of facia homogeneity and normalization of crystalloscopic rate for own crystallization (initiation potential for tezigraphic test).
We composed universal algorithm of the crystalloscopic monitoring of patient functional status, which consist of the two or three control points. If we estimate short-time or unitary medical interference, it is enough to investigate the baseline and final condition. In this time the taking of biosubstrates is accomplished. For the treatment scheme, which realized in long time, the three-points investigation is most suitable.
Conclusion. So, it was ascertained, that crystallographic methods of biofluids investigation can be used for the treatment control.
- Guliaeva S. F., Martusevich A. K., Pomaskina T. V. Mathematic modeling of saliva initiated crystallogenesis as a criteria of mineral water using effectiveness // Human Ecology. - 2005. - №7. - P. 33-35. (in Russian)
- Lovitz T. E. The demonstration of the new method of salt investigation // Technological Journal. - 1804. - Vol. 1. - Part 3. - P. 27-41. (in Russian)
- Martusevich A. K., Ghdanova O. B., Yanchenko V. A. Pathogenic role of biological fluids crystallization investigation on alveococcosis // Annals of Surgical Hepatology. - 2006. - Vol. 11, №3. - P. 50-51. (in Russian)
- Colomban Ph., Lasseguest J. S., Novak A. et al. Dynamics of molecular crystals. Amsterdam: Elsevier, 1991. - 269 p.
The article is admitted to the V International Scientific Conference «Present-day problems of experimental and clinical medicine», Thailand, (Pattaya), February 20-28, 2008, came to the editorial office on 03.11.07