Scientific journal
European Journal of Natural History
ISSN 2073-4972

LEVEL OF MONOCYTIC CHEMOATTRACTANT PROETEIN, TRANSFORMING GROWTH FACTOR β1, AND FIBROBLAST GROWTH FACTOR IN BLOOD SERUM OF PATIENTSWITH DIABETIC NEPHROPATHY

Bondyreva A.V. 1 Knyazeva I.L. 1 Stepchenko M.A. 1 Borisova N.A. 1
1 Kursk State Medical University

The objective of this work is to study a character of monocytic chemoattractant protein (MCP-1), transforming growth factor β1 (TGF-β1), and fibroblast growth factor (FGF) among patients with diabetic nephrpathy (DN).

Methods and materials. Two groups of patients with sugar diabetes of type 2 with different stages of DN, according to classification I.I. Dedov and M.V. Shestakova, 2000, were included into the investigation. Group 1 (n = 50) – with albuminuria, average level of microalbuminuria among patients of this group equaled 149,8 ± 5,2 mg/day. Group 2 (n = 35) – with proteinuria, daily proteinuria did not exceed 1,1 ± 0,3 g/day. Duration of SD of type 2 oscillated from 1 to 15 years among the studied. Average age equaled 41,0 ± 6,5 years. Blood samples of 20 healthy donors were used as a control. Serum concentration of МСР-1, TGF-β1, FGF was defined by the method of immunoferment analysis. Statistical processing of the received data was made with programme complex Statistica 8,0 for Windows.

Results and discussions. Defining contents of MCP-1 under DN has shown its increase 1,7 ± 0,2 times (р < 0,05) in proteinuric stage of DN, compared to the control (109,2 ± 4,1 pg/ml) and 1,3 ± 0,3 times (р < 0,05) – compared to the same index of albuminuric stage. Defining contents of FGF among patients with DN with albuminuric stage has established its reliable increase 1,5 ± 0,2 times (р < 0,05), compared to the control group (2,9 ± 1,1 pg/ml). The highest level of FGF,6,6 ± 2,4 pg/ml, was registered in blood serum of patients with proteinuric stage of DN. We have registered an increase in serum concentration of TGF-β1among patients with SD of type 2 with a progressing nephropathy. Thus, under proteinuric stage of DN level of TGF-β1equaled122,4 ± 3,5 pg/ml and was reliably higher than under albuminuric stage of DN (112,4 ± 3,6 pg/ml) and in the control group (40,9 ± 6,9 pg/ml).

Resume. An increase in serum level of МСР-1, TGF-β1, FGF, in a larger scale under proteinuric stage of disease, has been established under diabetic nephropathy.

The work was submitted to International Scientific Conference «Fundamental and applied research in medicine», France (Paris), 14-21, October, 2012, came to the editorial office on 18.10.2012.