Scientific journal
European Journal of Natural History
ISSN 2073-4972

EXPRESSION OF HLA-DRA,-DMA AND CIITA GENES IN BURN INJURY PATIENTS WITH SYSTEMIC INFLAMMATION RESPONSE SYNDROME

Burmistrova A.L., Koneva E.P., Suslova T.A., Shmunk I.V., Philippova J.J.

Severe thermal injury causes immune dysfunctions and frequently accompanied by developing of septic conditions, which characterized by decreased monocyte human leukocyte antigen-DR measurements.

Expression of HLA-DR on immune cells is regulated by specific genes. HLA-DM and CIITA genes have a strong influence on molecules of adaptive immune response. It is known, that genes of DM locus provides correct transport of HLA II molecules from endoplasmic reticulum and stable connection alpha-beta heterodimers with specific peptides. CIITA (class II transactivator) controls cellular specificity and expression of MHC II genes.

The aim of our research is to estimate expression of HLA-DRA,-DMA and CIITA genes in burn injury patients with SIRS-III (Systemic Inflammation Response Syndrome).

Patients and methods: Samples from 8 patients with burn injury and 10 healthy donors were investigated.

Expression of messenger RNA(mRNA) HLA-DRA, -DMA and CIITA genes was measured by quantitative polymerase chain reaction in real-time with reverse transcription (qRT-PCR real-time) using specific primers (Pachot A, 2005). Comparisons between groups were made using a nonparametric Mann-Whitney U test.

Results: We observed significant differences (р< 0,05) of genes (HLA-DRA, -DMA, CIITA) expression levels from patients and controls. Patients with severe burn injury had significant lower levels of genes expression in comparison with healthy donors. As genes HLA-DMA and CIITA have a strong influence on immune response (they are essential to ensure MHC II protein structure, transport, and peptide loading), their low expression in patients with severe thermal injury with SIRS-III could lead to deficient expression HLA-DR molecules on immune cells and could be a reason of developing immunosuppression. Decreasing expression levels of these genes can be used as a marker of developing immunodepression.

The work is submitted to the Scientific International Conference "Scientific Research of Higher School on Priority Orientations of Science and Technology", June, 13-20, 2008, Palermo-Savona, came to the editorial office on 22.04.2008.