The main aims this study are to estimate the adaptive mechanisms of the organism in developing cold trauma and investigate interleukin1 β (IL-1 β) and the factor of tumour necrosis α (TNF α) under the influence of potent irritant.
Materials and methods: the experiments were carried out on the male mature Vistar rats with the body weight 200-240gr. The models of frostbite induced by chlorethyl were employed within one week after damage. The withdrawal of rats was implicated by means of decapitation under etheric anesthesia in an hour, 24 hours, on fifth and seventh days. The approval of Ethics Board of the Northern state medical university was obtained. «The Regulations of the work with experimental animals » were observed according to the Enactment of Ministry of Health of the USSR №755 issued 12.08.1977.
The contents of the anti-inflammatory cytokine IL-1 β and TNF α was determined in blood serum with the help of immunofermental method using reagents manufactured by "RD Systems", USA. Experimental group was affected by cold. Control group consisted of intact animals. Statistical analysis was done on the basis of Statagraphcs plus 5.1 for Windows.
Results: the study demonstrated that the production of cytokine was dramatically decreased in the early reactive period (in one hour and 24 hours after damage) and sharply increased at the beginning of the late reactive period (at р ≤ 0,001). The imbalance of anti-inflammatory cytokine was observed at all experimental stages the secretion of TNF α prevailed. We suggested that in intact animals revealed cytokine performed a certain "sentry" function since it was this cytokine that was the mediator of « the first wave » responsible for triggering defensive immune mechanisms.
Conclusions: the secretion of cytokine IL-1 β and TNF α in a healthy organism reflects the current condition of immune system. Imbalance of cytokine at frostbite specifies indicates their role in pathogenesis of acute cryotrauma and may be of a certain value in diagnostics of the severity of injury in the monitoring cryotrauma and in developing new methods of treatment, which may prove to be more effective, than traditional ones.
The article is admitted to the International Scientific Conference " Innovation technologies in medicine"; Spain (Costa Brava) of the July 8-15, 2007г.; came to the editorial office on 28.07.07