Scientific journal
European Journal of Natural History
ISSN 2073-4972

RATS’ BLOOD PROTEOLYTIC ENZYMES’ ACTIVITY SEASONAL MANIFESTATIONS FEATURES

Sybanbekova D.R.

There is much enough material integrating the data about the role of proteolysis. The majority of these publications has a biochemical pattern in general and gives no comprehensive idea of the proteolysis value in chrono-physiological processes.

The purpose of the present work has been the study of the blood proteolytic enzymes´ activity seasonal dynamics.

White both sex non-pedigree rats weighing 200-270 g were used as the research objects. 3 sets of experiments with regularity of 10-15 days were carried out each season of the year. Every hour blood samplings were taken during the day using the decapitation method. In all the blood samples the level of general proteolytic activity of blood (GPA) and total protein amount of plasma were determined. The accumulation of free amino acids and peptides having NH2-groups in the probes incubated within 4 hours at 37С was considered to be the GPA level index. The findings were stated in mcg of glycin per 1 ml of plasma (or erythrocytes) for an hour of the incubation.

According to the research results the proteolytic blood system is the most active in the autumn-summer period of the year. The level of proteolytic activity of plasma enzymes in autumn makes 43,3±0,9-64,9±0,8 mcg Gly/ml/hour, in summer - 40,4±0,9-62,7±1,1 mcg Gly/ml/hour; the activity of erythrocyte proteases is 140,3±3,6-161,9±5,6 mcg Gly/ml/hour and 140,0±2,9-153,7±1,3 mcg Gly/ml/hour, accordingly. In winter and spring season the activity of proteolytic enzymes reduces considerably: in spring the level of plasma proteases makes 35,0±1,6-60,2±1,1 mcg Gly/ml/hour, in winter - 38,0±0,9-60,7±1,0 mcg Gly/ml/hour; the activity of erythrocyte proteases is 137,3±1,9-149,4±2,3 mcg Gly/ml/hour and 108,8±6-135,7±3,8 mcg Gly/ml/hour, accordingly. We have managed to determine a little difference in the dynamics of spring-winter manifestations of proteolysis activity in plasma and erythrocytes. In erythrocytes the proteolysis takes place more actively in spring, and in plasma - in winter.

The distribution of total protein amount indexes in blood plasma in different seasons of the year allowed determining the presence of the given parameter seasonal fluctuations. Thus, the total protein maximal level in plasma is registered in autumn and summer periods of the year: 73,4±3,8-95,3±4,3 g/l and 65,0±3,8-81,9±4,3 g/l, accordingly. A considerable protein reduction is registered in spring and winter periods: 51,03±0,4-73,4±1,1 g/l and 46,4±2,5-63,4±3,6 g/l, accordingly.

The article is admitted to the International Scientific Conference «Basic and applied research in medicine», China (Beijing), November 26 - December 4, 2007, came to the editorial office on 12.11.07