Scientific journal
European Journal of Natural History
ISSN 2073-4972

CYTOPLASMIC RNA CHANGES IN SKIN HAIR FOLLICLE EPITHELIAL CELLS OF GUINEA-PIGS AT MICROWAVE INFLUENCE

Melchikov A.S.

In the available literature there are no cytophotometric data about the cytoplasmatic RNA changes in skin hair follicles´ epithelial cells when affecting by thermogenous intensity microwaves. All this conditioned, especially with due account for the possibility of the obtained experimental data extrapolation for a human, the necessity to carry out our research.

The research was carried out on 65 mature guinea-pig males weighing 400-450 g, from which 35 were used in the experiment, and 30 served as the control. The experimental animals were exposed to the effect of single general microwave irradiation (length of wave - 12,6 сm, frequency - 2375 MHz,  power flow density (PFD) - 60 mW/cm2, exposure time - 10 min).  Excluding the animals from the experiment and sampling the materials were done immediately, in 6 hours, on the 1st, 5th, 10th, 25th and 60th days after finishing the exposure. . The flaps of skin were taken from different areas (head (cheek), back, stomach). The photometric activity definition of the cytoplasmic RNA content was performed in 50 cells of outer root sheaths of each cut hair follicles. The hematological control (total count of erythrocytes and leucocytes) was carried out during the experiment.

Right after finishing the microwave effect the decrease, compared to the control, of cytoplasmic RNA is registered, to the maximum extent - in the stomach skin epitheliocytes - up to 86,7%, while in the head and back skin - up to 98,3% and 97,2% from the original accordingly (р<0,05). In the following terms after finishing the exposure to the thermogenous intensity SHF waves a further decrease of the cytoplasmic RNA content in skin cells of all localizations, especially head and stomach, is observed. Thus, on the 5th day after finishing the SHF waves exposure the cytoplasmic RNA content is maximally decreased in the specified epitheliocytes of head and stomach skin - by 23,1% and 35,8% from the control level accordingly (p<0,05). Beginning with the 10th day an increase of the cytoplasmic RNA content in the cytoplasm of outer root sheaths epitheliocytes of all localizations skin hair follicles is close to the original one (p>0,05).

The article is admitted to the International Scientific Conference «Basic and applied problems of medicine and biology», Italy, Sicily, July 15-22, 2007, came to the editorial office on 1.11.07